Fast Blue RR—Siloxane Derivatized Materials Indicate Wound Infection Due to a Deep Blue Color Development

نویسندگان

  • Doris Schiffer
  • Gregor Tegl
  • Robert Vielnascher
  • Hansjoerg Weber
  • Rainer Schoeftner
  • Herfried Wiesbauer
  • Eva Sigl
  • Andrea Heinzle
  • Georg M. Guebitz
چکیده

There is a strong need for simple and fast methods for wound infection determination. Myeloperoxidase, an immune system-derived enzyme was found to be a suitable biomarker for wound infection. Hence, alkoxysilane-derivatized Fast Blue RR was immobilized via simple hydrolytic polymerization. The resulting enzyme-responsive siloxane layers were incubated with myeloperoxidase, wound fluid or hemoglobin. The reaction was monitored via HPLC measurements and the color development quantified spectrophotometrically. Myeloperoxidase was indeed able to oxidize immobilized Fast Blue RR leading to a blue colored product. No conversion was detected in non-infected wound fluids. The visible color changes of these novel materials towards blue enable an easy distinction between infected and non-infected wound fluids.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Deep blue-emissive bifunctional (hole-transporting + emissive) materials with CIEy 0.06 based on a U-shaped phenanthrene scaffold for application in organic light-emitting diodes

Introduction Development of deep blue-emissive materials (CIEy o 0.10) with improved physical as well as electroluminescence properties is of tremendous contemporary interest in organic light-emitting diodes (OLEDs). It is due to the fact that deep blue-emissive materials are more energy efficient than sky blue-emissive materials, and can be utilized as hostmaterials to produce any color by a c...

متن کامل

Fabrication and study of color and physical properties for cobalt silicate-titanite ceramic pigment by sol-gel method

Cobalt-based blue ceramic pigments are widely used in ceramic industries due to characteristics such as temperature stability, chemical stability and high resistance to these coatings. In this research, the blue silicate - titanate cobalt ceramic pigment is synthesized by sol-gel method and the dispersed pigments were printed on the ceramic by silk screen printing method in the industrial produ...

متن کامل

The effect of color type on early wound healing in farmed mink (Neovison vison)

BACKGROUND Individual differences of mink, including color type, are speculated to affect the course of wound healing, thereby impacting wound assessment and management on the farms, as well as the assessment of wounds in forensic cases. In this study, we examined the effect of color type on early wound healing in farmed mink. Full thickness excisional wounds (2 × 2 cm) were made on the back in...

متن کامل

ar X iv : a st ro - p h / 05 12 12 7 v 1 5 D ec 2 00 5 Galaxies in SDSS and DEEP 2 : a quiet life on the blue sequence ?

In the six billion years between redshifts z = 1 and z = 0.1, galaxies change due to the aging of their stellar populations, the formation of new stars, and mergers with other galaxies. Here I explore the relative importance of these various effects, finding that while mergers are likely to be important for the red galaxy sequence they are unlikely to affect more than 10% of the blue galaxy seq...

متن کامل

The Evaluation of Yellow vs. Blue Color Contrast Sensitivity pre and Post LASIK

Introduction: contrast sensitivity is one of the most important psychophysical tests that may be used for the evaluation of refractive state of eye and retinal image quality. Yellow vs. blue color contrast sensitivity may be more suitable in this regard. Materials and Methods: thirty myopic eyes were considered in this study. Yellow vs. blue color contrast sensitivity was evaluated under the sa...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 8  شماره 

صفحات  -

تاریخ انتشار 2015